PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Arabidopsis thaliana
GeBP Family
Species TF ID Description
AT1G11510.1DNA-binding storekeeper protein-related transcriptional regulator
AT1G44810.1DNA-binding storekeeper protein-related transcriptional regulator
AT1G55950.1DNA-binding storekeeper protein-related transcriptional regulator
AT1G61730.1DNA-binding storekeeper protein-related transcriptional regulator
AT1G66420.1DNA-binding storekeeper protein-related transcriptional regulator
AT2G01370.1DNA-binding storekeeper protein-related transcriptional regulator
AT2G20613.1DNA-binding storekeeper protein-related transcriptional regulator
AT2G25650.1DNA-binding storekeeper protein-related transcriptional regulator
AT2G36340.1DNA-binding storekeeper protein-related transcriptional regulator
AT3G04930.1DNA-binding storekeeper protein-related transcriptional regulator
AT3G04930.2DNA-binding storekeeper protein-related transcriptional regulator
AT4G00130.1DNA-binding storekeeper protein-related transcriptional regulator
AT4G00232.1DNA-binding storekeeper protein-related transcriptional regulator
AT4G00238.1DNA-binding storekeeper protein-related transcriptional regulator
AT4G00250.1DNA-binding storekeeper protein-related transcriptional regulator
AT4G00270.1DNA-binding storekeeper protein-related transcriptional regulator
AT4G00390.1DNA-binding storekeeper protein-related transcriptional regulator
AT4G00610.1DNA-binding storekeeper protein-related transcriptional regulator
AT4G01260.1DNA-binding storekeeper protein-related transcriptional regulator
AT4G25210.1DNA-binding storekeeper protein-related transcriptional regulator
AT5G14280.1DNA-binding storekeeper protein-related
AT5G28040.1DNA-binding storekeeper protein-related transcriptional regulator
AT5G41765.1DNA-binding storekeeper protein-related transcriptional regulator
GeBP Family Introduction

Understanding the role of transcription factors (TFs) is essential in reconstructing developmental regulatory networks. The plant-specific GeBP TF family of Arabidopsis thaliana (Arabidopsis) comprises 21 members, all of unknown function. A subset of four members, the founding member GeBP and GeBP-like proteins (GPL) 1, 2, and 3, shares a conserved C-terminal domain. Here we report that GeBP/GPL genes represent a newly defined class of leucine-zipper (Leu-zipper) TFs and that they play a redundant role in cytokinin hormone pathway regulation. Specifically, we demonstrate using yeast, in vitro, and split-yellow fluorescent protein in planta assays that GeBP/GPL proteins form homo- and heterodimers through a noncanonical Leu-zipper motif located in the C-terminal domain. A triple loss-of-function mutant of the three most closely related genes gebp gpl1 gpl2 shows a reduced sensitivity to exogenous cytokinins in a subset of cytokinin responses such as senescence and growth, whereas root inhibition is not affected. We find that transcript levels of type-A cytokinin response genes, which are involved in the negative feedback regulation of cytokinin signaling, are higher in the triple mutant. Using a GPL version that acts as a constitutive transcriptional activator, we show that the regulation of Arabidopsis response regulators (ARRs) is mediated by at least one additional, as yet unknown, repressor acting genetically downstream in the GeBP/GPL pathway. Our results indicate that GeBP/GPL genes encode a new class of unconventional Leu-zipper TF proteins and suggest that their role in the cytokinin pathway is to antagonize the negative feedback regulation on ARR genes to trigger the cytokinin response.

Chevalier F, Perazza D, Laporte F, Le Henanff G, Hornitschek P, Bonneville JM, Herzog M, Vachon G
GeBP and GeBP-Like Proteins Are Noncanonical Leucine-Zipper Transcription Factors That Regulate Cytokinin Response in Arabidopsis
Plant Physiol. 2008 Mar;146(3):1142-54.
PMID: 18162594