PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Arabidopsis lyrata
HSF Family
Species TF ID Description
321853HSF family protein
349389HSF family protein
353697HSF family protein
356702HSF family protein
473420HSF family protein
473862HSF family protein
477093HSF family protein
477545HSF family protein
479794HSF family protein
479964HSF family protein
481471HSF family protein
485548HSF family protein
488541HSF family protein
490909HSF family protein
492972HSF family protein
493100HSF family protein
493513HSF family protein
494414HSF family protein
495484HSF family protein
858789HSF family protein
859202HSF family protein
875173HSF family protein
876867HSF family protein
894128HSF family protein
901783HSF family protein
908246HSF family protein
908564HSF family protein
909257HSF family protein
911518HSF family protein
913148HSF family protein
914699HSF family protein
914704HSF family protein
914765HSF family protein
914975HSF family protein
914977HSF family protein
915131HSF family protein
918562HSF family protein
919451HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645