PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Azadirachta indica
FAR1 Family
Species TF ID Description
Neem_10025_f_1FAR1 family protein
Neem_10098_f_2FAR1 family protein
Neem_10146_f_2FAR1 family protein
Neem_11021_f_3FAR1 family protein
Neem_11663_f_1FAR1 family protein
Neem_11891_f_2FAR1 family protein
Neem_11891_f_3FAR1 family protein
Neem_15768_f_1FAR1 family protein
Neem_15944_f_1FAR1 family protein
Neem_1766_f_2FAR1 family protein
Neem_20008_f_1FAR1 family protein
Neem_21061_f_1FAR1 family protein
Neem_21187_f_2FAR1 family protein
Neem_22186_f_1FAR1 family protein
Neem_23265_f_2FAR1 family protein
Neem_24113_f_1FAR1 family protein
Neem_2431_f_1FAR1 family protein
Neem_25415_f_1FAR1 family protein
Neem_2595_f_6FAR1 family protein
Neem_2827_f_2FAR1 family protein
Neem_2911_f_1FAR1 family protein
Neem_29285_f_1FAR1 family protein
Neem_3034_f_1FAR1 family protein
Neem_3037_f_1FAR1 family protein
Neem_31666_f_1FAR1 family protein
Neem_32037_f_1FAR1 family protein
Neem_323_f_14FAR1 family protein
Neem_35572_f_1FAR1 family protein
Neem_35723_f_2FAR1 family protein
Neem_35723_f_3FAR1 family protein
Neem_36966_f_1FAR1 family protein
Neem_3898_f_1FAR1 family protein
Neem_3898_f_2FAR1 family protein
Neem_3898_f_3FAR1 family protein
Neem_3991_f_2FAR1 family protein
Neem_41287_f_2FAR1 family protein
Neem_41398_a_1FAR1 family protein
Neem_4164_f_1FAR1 family protein
Neem_41784_f_1FAR1 family protein
Neem_4442_f_3FAR1 family protein
Neem_4451_f_1FAR1 family protein
Neem_5079_f_3FAR1 family protein
Neem_5444_f_3FAR1 family protein
Neem_586_f_4FAR1 family protein
Neem_6812_f_1FAR1 family protein
Neem_7230_f_2FAR1 family protein
Neem_833_f_10FAR1 family protein
Neem_833_f_14FAR1 family protein
Neem_833_f_7FAR1 family protein
Neem_833_f_8FAR1 family protein
Neem_833_f_9FAR1 family protein
Neem_854_f_6FAR1 family protein
Neem_8611_f_1FAR1 family protein
Neem_8730_f_1FAR1 family protein
Neem_915_f_4FAR1 family protein
Neem_9164_f_1FAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885