PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Actinidia chinensis
HSF Family
Species TF ID Description
Achn029931HSF family protein
Achn057551HSF family protein
Achn081581HSF family protein
Achn086291HSF family protein
Achn091321HSF family protein
Achn104121HSF family protein
Achn124291HSF family protein
Achn126231HSF family protein
Achn131591HSF family protein
Achn145961HSF family protein
Achn155631HSF family protein
Achn159441HSF family protein
Achn167241HSF family protein
Achn180221HSF family protein
Achn181551HSF family protein
Achn182081HSF family protein
Achn247541HSF family protein
Achn266121HSF family protein
Achn287331HSF family protein
Achn313391HSF family protein
Achn318511HSF family protein
Achn322761HSF family protein
Achn322771HSF family protein
Achn338111HSF family protein
Achn340021HSF family protein
Achn358341HSF family protein
Achn358711HSF family protein
Achn359401HSF family protein
Achn367271HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645