PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v1.0, v2.0, v3.0
Actinidia chinensis
FAR1 Family
Species TF ID Description
Achn000081FAR1 family protein
Achn000821FAR1 family protein
Achn002281FAR1 family protein
Achn002531FAR1 family protein
Achn012651FAR1 family protein
Achn014121FAR1 family protein
Achn018201FAR1 family protein
Achn019771FAR1 family protein
Achn020651FAR1 family protein
Achn030321FAR1 family protein
Achn032341FAR1 family protein
Achn044611FAR1 family protein
Achn046071FAR1 family protein
Achn058751FAR1 family protein
Achn065071FAR1 family protein
Achn065501FAR1 family protein
Achn067421FAR1 family protein
Achn069111FAR1 family protein
Achn071481FAR1 family protein
Achn074601FAR1 family protein
Achn078441FAR1 family protein
Achn078551FAR1 family protein
Achn088571FAR1 family protein
Achn093351FAR1 family protein
Achn094131FAR1 family protein
Achn097861FAR1 family protein
Achn100281FAR1 family protein
Achn128261FAR1 family protein
Achn139261FAR1 family protein
Achn154831FAR1 family protein
Achn156891FAR1 family protein
Achn158101FAR1 family protein
Achn163291FAR1 family protein
Achn164231FAR1 family protein
Achn165991FAR1 family protein
Achn173641FAR1 family protein
Achn176661FAR1 family protein
Achn176741FAR1 family protein
Achn204981FAR1 family protein
Achn205991FAR1 family protein
Achn209551FAR1 family protein
Achn210321FAR1 family protein
Achn211241FAR1 family protein
Achn211251FAR1 family protein
Achn213491FAR1 family protein
Achn224681FAR1 family protein
Achn231181FAR1 family protein
Achn232491FAR1 family protein
Achn236831FAR1 family protein
Achn237121FAR1 family protein
Achn242741FAR1 family protein
Achn247721FAR1 family protein
Achn250031FAR1 family protein
Achn253161FAR1 family protein
Achn258341FAR1 family protein
Achn263121FAR1 family protein
Achn264271FAR1 family protein
Achn264411FAR1 family protein
Achn267741FAR1 family protein
Achn272241FAR1 family protein
Achn275271FAR1 family protein
Achn275581FAR1 family protein
Achn284071FAR1 family protein
Achn287391FAR1 family protein
Achn290481FAR1 family protein
Achn292641FAR1 family protein
Achn310101FAR1 family protein
Achn314731FAR1 family protein
Achn314811FAR1 family protein
Achn317851FAR1 family protein
Achn317861FAR1 family protein
Achn324281FAR1 family protein
Achn325861FAR1 family protein
Achn327871FAR1 family protein
Achn327891FAR1 family protein
Achn327901FAR1 family protein
Achn337331FAR1 family protein
Achn343591FAR1 family protein
Achn345551FAR1 family protein
Achn346681FAR1 family protein
Achn360501FAR1 family protein
Achn370431FAR1 family protein
Achn372231FAR1 family protein
Achn376681FAR1 family protein
Achn376871FAR1 family protein
Achn377091FAR1 family protein
Achn377111FAR1 family protein
Achn391061FAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885